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broth microdilution method (clsi, 2012)  (Clinical and Laboratory Standards Institute)

 
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    Clinical and Laboratory Standards Institute broth microdilution method (clsi, 2012)
    Broth Microdilution Method (Clsi, 2012), supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clsi+broth+microdilution+method/disk+diffusion+method/pm40107472-208-21-24
    Average 90 stars, based on 1 article reviews
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    other:

    Article Title: Purification and characterization of bacteriocins-like inhibitory substances from food isolated Enterococcus faecalis OS13 with activity against nosocomial enterococci
    Article Snippet: In order to perform antibiotic susceptibility typing of the 11 putative bacteriocinogenic E. faecalis isolates to check whether they are relevant to each other or from different clones, and to overview their biosafety for further applications, the Clinical and Laboratory Standards Institute (CLSI) broth microdilution method in microtiter plates was used to determine the antibiotic resistance phenotype and MIC of these isolates against 13 antibiotics with different concentration ranges (Table ), using Mueller–Hinton broth (Oxoid).

    Article Title: Invasive pneumococcal disease surveillance in Canada, 2020
    Article Snippet: Minimum inhibitory concentrations were determined by the Clinical and Laboratory Standards Institute (CLSI) broth microdilution method using 96-well custom designed microtitre plates prepared by Canadian Antimicrobial Resistance Alliance ( (( 18 ))).

    Article Title: Antimicrobial and antioxidant potential of Yemeni Sidr honey against multidrug-resistant pathogens – in vitro and in silico studies
    Article Snippet: The MIC illustrated in (Table 2) was obtained by utilizing the standard Clinical and Laboratory Standards Institute (CLSI) broth microdilution method [22].

    Article Title: Novel Gut Microbiota Modulator, Which Markedly Increases Akkermansia muciniphila Occupancy, Ameliorates Experimental Colitis in Rats.
    Article Snippet: Background Since gut microbiota is involved in the pathogenesis of inflammatory bowel disease (IBD), antibiotics or probiotics may be attractive options for the treatment of IBD.. Akkermansia muciniphila is expected as a next-generation probiotic for IBD, and OPS-2071 is a novel quinolone with potent antibacterial activity against Clostridioides difficile.. Aims The aim of this study is to assess the potential of OPS-2071 as a gut microbiota modulator for IBD.

    Article Title: Synergistic activity of weak organic acids against uropathogens.
    Article Snippet: Minimum inhibitory concentration (MIC) values of WOAs against P. mirabilis, S. aureus, E. coli and P. aeruginosa were determined according to the CLSI (Clinical and Laboratory Standards Institute) broth microdilution method [21].

    In Vitro:

    Article Title: Comparative evaluation of sensititre YeastOne and VITEK2 antifungal susceptibility tests with CLSI broth microdilution method of clinical Cryptococcus isolates in Taiwan.
    Article Snippet: .. TABLE 1 In vitro antifungal susceptibilities of 44 C. neoformans isolates determined by the CLSI broth microdilution method, Sensititre YeastOne, and VITEK2 Susceptibility Carda Drug MIC (μg/mL) Non-WT Non-WT Method <0.03 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 (CLSI) (EUCAST) AMB BMD 33 11 11 0 SYO 1 (<0.12) 2 33 8 8 0 5FC BMD 2 20 22 0 SYO 10 29 4 1 1 VITEK2 17 (≤1) 27 0 FLC BMD 1 1 8 28 6 0 SYO 2 4 33 3 2 5 VITEK2 1 (≤0.5) 3 40 0 VRC BMD 2 13 25 4 0 0 SYO 1 (0.015) 1 22 18 2 0 0 VITEK2 43 (≤0.12)b 0 0 ITC BMD 12 9 16 7 0 SYO 2 26 14 2 2 PSC BMD 1 3 30 10 0 0 SYO 8 33 3 3 0 ISC BMD 2 13 21 6 2 MGX BMD 2 1 8 24 8 1 aCLSI, Clinical and Laboratory Standards Institute; WT, wild type; EUCAST, European Committee on Antimicrobial Susceptibility Testing; BMD, broth microdilution; SYO, Sensititre YeastOne; AMB, amphotericin B; 5FC, flucytosine; FLC, fluconazole; VRC, voriconazole; ITC, itraconazole; PSC, posaconazole; ISC, isavuconazole; MGX, manogepix. bMIC to VRC was undetermined in one isolate by VITEK2 due to insufficient growth in positive control well despite repeated testing for three times. ..

    Positive Control:

    Article Title: Comparative evaluation of sensititre YeastOne and VITEK2 antifungal susceptibility tests with CLSI broth microdilution method of clinical Cryptococcus isolates in Taiwan.
    Article Snippet: .. TABLE 1 In vitro antifungal susceptibilities of 44 C. neoformans isolates determined by the CLSI broth microdilution method, Sensititre YeastOne, and VITEK2 Susceptibility Carda Drug MIC (μg/mL) Non-WT Non-WT Method <0.03 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 (CLSI) (EUCAST) AMB BMD 33 11 11 0 SYO 1 (<0.12) 2 33 8 8 0 5FC BMD 2 20 22 0 SYO 10 29 4 1 1 VITEK2 17 (≤1) 27 0 FLC BMD 1 1 8 28 6 0 SYO 2 4 33 3 2 5 VITEK2 1 (≤0.5) 3 40 0 VRC BMD 2 13 25 4 0 0 SYO 1 (0.015) 1 22 18 2 0 0 VITEK2 43 (≤0.12)b 0 0 ITC BMD 12 9 16 7 0 SYO 2 26 14 2 2 PSC BMD 1 3 30 10 0 0 SYO 8 33 3 3 0 ISC BMD 2 13 21 6 2 MGX BMD 2 1 8 24 8 1 aCLSI, Clinical and Laboratory Standards Institute; WT, wild type; EUCAST, European Committee on Antimicrobial Susceptibility Testing; BMD, broth microdilution; SYO, Sensititre YeastOne; AMB, amphotericin B; 5FC, flucytosine; FLC, fluconazole; VRC, voriconazole; ITC, itraconazole; PSC, posaconazole; ISC, isavuconazole; MGX, manogepix. bMIC to VRC was undetermined in one isolate by VITEK2 due to insufficient growth in positive control well despite repeated testing for three times. ..

    Concentration Assay:

    Article Title: Efficient Production of Broad-Spectrum Antimicrobials by Paenibacillus polymyxa OSY-EC Using Acid Whey-Based Medium and Novel Antimicrobial Concentration Approach.
    Article Snippet: The targeted fungal strains were Candia albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404. .. The minimum inhibitory concentration (MIC) of the antimicrobials against the targeted strains was determined using the standard CLSI broth microdilution method (Clinical and Laboratory Standards Institute, 2015), as described later. ..



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    Image Search Results


    Results of DNA barcoding and susceptibility tests Species were confirmed by DNA barcoding using nucleotide sequences of internal transcribed spacer (ITS: ITS1/ITS4 primer [ <xref ref-type= 5 ]) small subunit region (SSU: NS1/NS2 primer [ 5 ]) and large subunit region (LSU: NL-1/NL-4 primer [ 6 ]) of the ribosomal DNA gene. The purified PCR products were then outsourced for Sanger sequencing service (First BASE Laboratories, Singapore Science Park II, Singapore). The sequencing results were subjected to BLASTN (National Center for Biotechnology Information Internet homepage) search against the GenBank database for homology identities. Antifungal susceptibility patterns of the mold against common antifungal medications were assessed by the broth dilution technique (according to the Clinical and Laboratory Standards Institute (CLSI) document M38-A2 protocol) with reference to Aspergillus spp . AMB, amphotericin B; CAS, caspofungin; FLC, fluconazole; ISZ, isavuconazole; ITZ, itaconazole; PSZ, Posaconazole; TBF=terbinafine; VCZ, voriconazole; (R), resistance; (S), susceptible; (N/A), non-available" width="100%" height="100%">

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    doi: 10.7759/cureus.57016

    Figure Lengend Snippet: Results of DNA barcoding and susceptibility tests Species were confirmed by DNA barcoding using nucleotide sequences of internal transcribed spacer (ITS: ITS1/ITS4 primer [ 5 ]) small subunit region (SSU: NS1/NS2 primer [ 5 ]) and large subunit region (LSU: NL-1/NL-4 primer [ 6 ]) of the ribosomal DNA gene. The purified PCR products were then outsourced for Sanger sequencing service (First BASE Laboratories, Singapore Science Park II, Singapore). The sequencing results were subjected to BLASTN (National Center for Biotechnology Information Internet homepage) search against the GenBank database for homology identities. Antifungal susceptibility patterns of the mold against common antifungal medications were assessed by the broth dilution technique (according to the Clinical and Laboratory Standards Institute (CLSI) document M38-A2 protocol) with reference to Aspergillus spp . AMB, amphotericin B; CAS, caspofungin; FLC, fluconazole; ISZ, isavuconazole; ITZ, itaconazole; PSZ, Posaconazole; TBF=terbinafine; VCZ, voriconazole; (R), resistance; (S), susceptible; (N/A), non-available

    Article Snippet: Minimum inhibitory concentrations (MIC) values for various antifungal agents were determined using the modified Clinical and Laboratory Standards Institute (CLSI) M38-A broth microdilution method [ ] with reference to Aspergillus spp [ ].

    Techniques: Purification, Sequencing, Medications, Activity Assay, Infection